ID-Blood – A lateral flow test for rapid and visual blood group genotyping
The haemagglutination is the standard method for the determination of red blood cell phenotype. However, this serological technique has some limitations, especially in certain clinical situations (polytransfused patients, presence of autoantibodies…) where the patient’s phenotype cannot be determined. In these cases, the determination of the genotype by molecular biology, for the clinically relevant antigens, could overcome these difficulties by providing patient’s phenotype predicted from the genotype, and to prevent the risk of red blood cell alloimmunization.
To overcome these limitations, we developed a rapid and visual test allowing simultaneous determination of the genotype of the six clinically relevant blood antigens (JK1, JK2, FY1, FY2, MNS3, MNS4) for the management of patient requiring transfusion, in order to predict the extended-antigen phenotype. Our patented technology (PCT/FR2016052272) is based on the co-amplification of multiple regions of gDNA and the use of a lateral-flow test for visual reading of results with naked-eye.
A compléter par Daisy